Diagnostic efficiency of P63/SMA double labeled immunochemistry in lesion tissues of breast cancer
WANG Yong-jun
LYN Fei
WANG Xiao-xiao
WANG Ling
DING Chun-yan
FAN Xiao-jie
YANG Hui-chai
LIU Yue-ping
Abstract：Objective To investigate the diagnostic efficiency of P63/smooth muscle actin ( SMA) double labeled im-munochemistry in lesion tissues of breast cancer.Methods A total of 150 cases of patients with breast disease were select-ed as the subjects.Needle aspiration was performed to attain the breast specimens.HE staining and double labeled immu-nohistochemical staining of P63 and SMA were used.The results were observed and analyzed by using light microscope. Results Both P63 and SMA were positive in the vast majority of breast myoepithelial cells and P63 was negative in non-myoepithelial cells.Thirty-two cases by HE staining and 20 cases by double labeled immunohistochemical staining were di-agnosed with benign breast lesions, respectively;and 119 cases by HE staining and 121 cases by double labeled immuno-histochemical staining of suspicious carcinoma cells or carcinoma cell were found.When we compared the pathological re-sults, the accuracy rates in diagnosis of benign breast lesions with HE staining and double labeled immunohistochemical staining were 91%and 97%, respectively.The diagnostic accuracy rates of malignant breast lesions were 100% by both HE and double labeled immunohistochemical staining.Conclusion P63 and SMA double labeled immunohistochemical staining has higher accuracy rate in diagnosis of breast cancer and it could effectively diagnose the benign breast lesions.
Keywords：breast neoplasmsbreast carcinomadiagnostic testsimmunohistochemistryP63 proteinsmooth mus-cle actin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 14-16 )