Preparation of nano-crystalline of oridonin and its effect on cell proliferation and apoptosis of colorectal cancer cells in vitro
WANG Yong-qiang
JIN Hei-ying
LI Li-mei
WANG Shui-ming
WU Chuang
Abstract：Objective To prepare the nano-crystalline of oridonin and to investigate the effect on the proliferation and apoptosis of colorectal cancer cells.Methods The contents of oridonin nanosuspensions were prepared by high pressure homogenization.The nano-crystalline of oridonin was obtained after adding 5% mannitol, and then shaking and lyophili-zing.The solubility was determined in vitro.Colorectal cancer cells ( Lovo cells, SW480 cells, HCT-116 cells and HCT-8 cells) were cultured in vitro and incubated with different concentrations of nano-crystalline of oridonin (0, 5, 10, 15 and 25 μg/mL) .Cell proliferation was detected by cytometry.The apoptosis and cell cycle of cultured cells in different concen-trations of nano-crystalline of oridonin (5, 10 and 25 μg/mL) were detected.Results The nanosuspensions and nano-crystalline of oridonin was prepared successfully.There were significant differences in the proliferation inhibition rate among four kinds of colorectal cancer cells that were intervened by different concentrations of nano-crystalline of oridonin for 24 h and 72 h ( all P<0.05 ) .The nano-crystalline of oridonin with concentration of 25 μg/mL had the strongest inhibitory effect on cell proliferation as compared with the others (all P<0.05).The nano-crystalline of oridonin could block cell cy-cle of the Lovo cells and HCT-8 cells at G1 phase, and block the HCT-16 and SW480 cell cycle at S phase.Conclusion The vitro dissolution of nano-crystalline of oridonin is higher than oridonin.The nano-crystalline of oridonin can significant-ly inhibit the proliferation, induce the apoptosis and block the cell cycle of four kinds of colorectal cancer cells.
Keywords：oridoninnano-crystalline of oridoninhuman colorectal cancer cellscell proliferationapoptosiscell cyclein vitro experiment
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-7 )