Clinical characteristics of acute myeloid leukemia with FLT3-ITD and (or) FLT3-TKD mutation

TANG Ke-jing; WEI Hui; LIN Dong; LIU Yun-tao; ZHOU Chun-lin; WANG Ying; MI Ying-chang; WANG Jian-xiang; WANG Min

doi:10.3969/j.issn.1002-266X.2015.015.001

Abstract：Objective To observe the concurrence of acute myeloid leukemia ( AML) patients with FLT3 mutation-in-ternal tandem duplication ( FLT3-ITD) mutation and point mutation in the tyrosine kinase domain ( FLT3-TKD) , and then to analyze and compare the clinical characteristics between patients with the double mutations and patients with single muta-tion.Methods A total of 432 patients with AML were selected.We obtained the fresh bone marrow to separate the mono-nuclear cells and extract DNA.The amplifications of FLT3 gene exons 14, 15 multiple mutation domains and exon 20 muta-tion domain was conducted by PCR.ITD and TKD mutations were analyzed by agarose gel electrophoresis (AGE).The clinical data of patient′s gender, age, white blood cells, hemoglobin, platelets, primitive cell percentages of peripheral blood and bone marrow, and remission in AML patients with both FLT3-ITD and FLT3-TKD mutations and patients with single mutation were collected and compared.Results Among 432 AML patients, 102 patients with FLT3 mutation were found, and 11 AML patients with concurrence of both FLT3-ITD and FLT3-TKD mutations;and the other 91 AML patients with only one type of FLT3 mutation, including 60 with FLT3-ITD and 31 with FLT3-TKD.There were no significant differ-ences in the gender, age, white blood cells, hemoglobin, platelets, primitive cell percentages of peripheral blood and bone marrow, and complete remission between AML patients with both FLT3-ITD and FLT3-TKD mutations and AML patients with only one mutation.Conclusions There are a few AML patients with both FLT3-ITD and FLT3-TKD mutations.No significant differences are found in the clinical feathers between patients with both types of FLT3 mutations and patients car-rying only one kind of FLT3 mutation.