Effect of metallothionein 2A silencing on Cpy6B2 expression of breast cancer cells
WEI Xi-jun
WANG Qing-lu
LI Qiao-qiao
JIANG Xiao-hua
Abstract：Objective To investigate the effect of metallothionein 2A ( MT2A) silencing on cytochrome C oxidase 6B2 subunit (Cpy6B2) expression of breast cancer cells .Methods The breast cancer wild cell line MCF-7 (hereinafter referred as the MCF-7) was divided into the blank control group and MT 2A interference group, and each group had 12 holes.The blank control group did not transfect any genetic materials , and the MT2A interference group transfected the shRNA-MT2A vector.Using semi-quantitative PCR to detect the MT2A mRNA expression levels of the two groups .The MT2A interference group successfully obtained the MT 2A gene silencing .The real-time quantitative PCR was used to ana-lyze the expression of Cpy6B2 in the two groups.Results The results showed that the expression level of MCF-7 Cpy6B2 was increased under the MT2A gene silencing.Conclusion MT2A can inhibit the Cpy6B2 expression of breast cancer cells.
Keywords：metallothioneinbreast cancer cellscytochrome C oxidase 6B2 subunit
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 19-21 )