Expression changes and significance of TGF-βand MMPs in arterial tissues of rats with arterial calcification
WEI Xiao-min
QIN Xiao
LU Hai-lin
JIANG Wen-hong
Abstract：Objective To explore the potential role of transforming growth factor-β( TGF-β) and matrix metallopro-teinases ( MMPs) in its occurrence and development of arterial calcification .Methods Sixty male SD rats were randomly divided into the control group and experimental group , with 30 rats in each group .Rats in the experimental group were in-jected vitamin D3 with a dose of 500 000 U/( kg· d) subcutaneously for 3 consecutive days , which was in order to induce the arterial calcification in rat models .The same dose of 5% glucose without vitamin D3 was injected into the rats of two groups subcutaneously .Using Von Kossa staining to observe the degree of tissue calcification of the rats , and using real-time fluorescent quantitative PCR to determinate the expression of mRNA of TGF-βand MMPs in the two groups .Results The degree of calcification and the mRNA expression of TGF-βand MMPs in the experimental group were significantly higher than those of the control group (all P<0.05).Conclusion The TGF-βand MMPs may contribute to the formation of arterial calcification .
Keywords：arterial calcificationmatrix metalloproteinasesmatrix metalloproteinases-9transforming growth factor-β
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 16-18 )