Inhibitory effect of pIRES-scFvCD44-TRAIL on breast cancer cells

WEI Yang-hui; HUANG Yao; ZHANG Wei-xing

doi:10.3969/j.issn.1002-266X.2015.14.004

Abstract：Objective To investigate the inhibitory effects of CD 44 single-chain antibody scFvCD 44 and tumor necrosis factor-related apoptosis inducing ligand eukaryotic expression vector ( pIRES-scFvCD44-TRAIL ) on breast cancer cells MDA-MB-231.Methods ①Cellexperiments:TheplasmidDNAofpIRES-scFvCD44-TRAIL,whosegeneswereampli-fied by reverse transcriptase-polymerase chain reaction ( RT-PCR) , was transfected into breast cancer cells MDA-MB-231 ( the transfected group ) .The blank group was transfected with the blank pIRES plasmid and the negative group was not treated.After 24 hours, the apoptosis rate was calculated by flow cytometry .The scratch test was performed to observe the cell motility assay as well as to calculate the scratch healing rate .② Graft model experiments:Eight nude mice of breast cancer graft model were randomly divided into the experiment group and the control group .Mice in the experiment group re-ceived subcutaneous injection of pIRES-scFvCD44-TRAIL 100μL per rat, once two days, and for five times totally.Mice in the control group were treated by the same amount of normal saline .Tumor growth was observed , and the tumor inhibitory ratewascalculated.TheexpressionofKi67wasdetectedbyimmunohistochemistry.Results ①Cellexperiments:The transfected group had higher apoptosis rate and lower wound-healing rate as compared with the blank group and the negative group.②Graft model experiments:The tumor inhibitory rate of the observation group was 79.8%.The Ki67-positive rate of the observation group was significantly lower than that of the control group (P<0.05).Conclusion pIRES-scFvCD44-TRAIL can inhibit the growth and metastasis of breast cancer cells and its transplanted tumor .