Construction of HeLa stabIe expressing ceII Iine expressing FAM92A1-289 and its function on ceII proIiferation
GUI Hui
GUO Xing-rong
FANG Juan
DING Yan
SHEN Jun-hao
RUAN Xu-zhi
Abstract：Objective To construct the HeLa stable expressing cell line expressed by FAM92A1-289 and to observe its function on cell proliferation.Methods We constructed the recombinant expression vector CMV-SP6-TALEN-GFP-289, then transfected it into HeLa cells.We obtained the stable expressing cells HeLa/289 of FAM92A1-289 by puromycin screen-ing.Scratch assay colony-formation assay and MTT assay were used to detect the changes in migration, tumor growth rate and survival rate of HeLa/289 cells.Results The cervical cancer HeLa stable expressing cell line over-expressing FAM92A1-289 was successfully constructed.After detection, the migration ability was stronger, rate of growth was faster and the survival rate was higher.Conclusion The stable expressing cells HeLa/289 of FAM92A1-289 were established succesfully. FAM92A1-289 gene has the effect of promoting cell proliferation, and it is the related gene regulating the cell proliferation.
Keywords：uterine cervical neoplasmscell proliferationFAM92A1-289 gene
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-12 )