Effects of thyrotropin on expression IeveIs of Ieptin, adiponectin, TNF-αand IL-6 in rat adipose tissues
LAI Ya-xin
WANG Hao-yu
FAN Chen-ling
ZHANG Hong-mei
WANG Hong
TENG Wei-ping
SHAN Zhong-yan
Abstract：Objective To investigate the effects of thyroid stimulating hormone ( TSH) on the expression changes of leptin, adiponectin, tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) in the rat adipose tissues.Methods Twenty-four Wistar rats were randomly divided into three groups, and each had 8 rats.rats in the clinical hypothyroidism (CH) group received complete surgical removal of the thyroid and subcutaneous injection of 0.9% sodium chloride solu-tion;rats in the normal control ( C) group received thyroidectomy sham operation and subcutaneous injection of 0.9%sodi-um chloride solution;rats in the subclinical hypothyroidism ( SCH) group underwent surgical removal of the thyroid in the neck back, and then received daily subcutaneous injection of L-T4 solution to establish subclinical hypothyroidism models. Specimens from the visceral adipose tissues around epididymis were collected.Western blottingting and real-time PCR were used to detect the protein and mRNA expression levels of leptin, adiponectin, TNF-αand IL-6.Results The protein and mRNA expression levels of leptin, TNF-αand IL-6 were higher in the CH and SCH groups than those of the control group (all P<0.05), but the adiponectin expression was lower than that of the control group (all P<0.05).The expression levels of leptin, TNF-αand IL-6 were higher in the CH group, but the adiponectin expression was lower than that of the SCH group (all P<0.05).Conclusion TSH can promote the expression of leptin, TNF-αand IL-6 and inhibit the adi-ponectin expression in rat adipose tissues.
Keywords：adipose tissuethyroid stimulating hormoneleptinadiponectintumor necrosis factor-αinterleukin-6rats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )