Fabrication of BMSCs/BMP-2/calcined bone complex and its application in the repair of bone defect of rabbit ulna

ZHANG Bin; ZHAN Mei-sheng; ZHAO Yu-xi; WANG Wan-yin; YANG Jian-qiang; SHEN Jian-qiang; LIU Yun-hua; TANG Jin-bing; LI Yan-guo; WU Xuan

doi:10.3969/j.issn.1002-266X.2015.12.07

Abstract：Objective To make the rabbit bone marrow mesenchymal stem cells ( BMSCs )/bone morphogenetic pro-tein 2 (BMP2)/calcined bone complex, and to observe its application in the repair of bone defect of rabbit ulna .Methods After the isolation, culture and osteogenic induction of rabbit BMSCs , we inoculated on BMP2/calcined bone complex , and observed the compound situation under scanning electron microscope .Fifty-four New Zealand white rabbits were ran-domly divided into the experimental group , control group , and blank group with 18 rats in each group .Under the sterile conditions , 10 mm defect of the right middle ulna was made .The experimental group was implanted BMSCs/BMP2/cal-cined bone complexes , while the control group was implanted BMP 2/calcined bone complexes , and the blank group without any implantation.After the operation, we did not make any internal and external fixation .After operation 4, 8, and 12 weeks, 6 rabbits in each group were randomly sacrificed , and the radiological and histological examinations of the bone de-fects were conducted .Results The BMSCs cells after the osteogenic induction uniformly distributed in the bone surface and hole, and calcined bone surface attached closely , cells were round with smooth surface , large cell body and the diame-ter of 15 μm.With the extension of time , the Lane-Sandhu radiological score and histological score of the experimental group and the control group were increased gradually (all P<0.05);the blank group had no change (P>0.05).Four, eight and twelve weeks after implantation , the Lane-Sandhu radiological score and histological score of the experimental group and the control group were higher than those of the control group , and the experimental group increased more signifi-cantly (all P<0.05).Conclusion BMSCs can co-culture with BMP2/calcined bone complexes better in vitro , and the implantation of BMSCs/BMP2/calcined bone complexes help the repair of rabbit ulna bone defect .