Effect of propofol on expression of Homer-1 a in cerebral ischemia reperfusion rats and the mechanism

SHEN Da-chuan; DONG Bin; WEN Chao

doi:10.3969/j.issn.1002-266X.2015.12.004

Abstract：Objective To observe the effect of propofol on the expression of Homer-1a in the brain tissues of cerebral ischemia reperfusion rats , and to explore its mechanism .Methods A total of 120 male SD rats were randomly divided into the sham operation group , model group and propofol group , 40 rats in each group .Cerebral ischemia reperfusion rat models were established in the model group and propofol group by using the suture method ; sham operation group only received vascular separation processing , without line bolt insertion .Thirty min after modeling , rats in the propofol group were given propofol 10 mg/100 g by intraperitoneal injection , while rats in the sham operation and control groups were injected the same amount of normal saline .After the drug treatment for 3 h, 24 h, 72 h and 7 d, neurological function score was evalu-ated, Homer-1a mRNA level in the brain tissues was detected by using the real-time PCR, and S-100βin the peripheral blood was detected by enzyme linked immunosorbent assay .Results Compared with the sham operation group , the neuro-logical function scores and S-100βlevels of peripheral blood were increase at the four time points in the model and propofol groups (all P<0.05), and the model group increased more significantly (all P<0.05).Compared with the sham opera-tion group, the level of Homer-1a mRNA decreased in the model group at the time points of 3 h and 24 h (all P<0.05). The Homer-1a mRNA level of the propofol group was higher than those in the other two groups at the four time points ( all P<0.05).In all rat samples, Homer-1a mRNA expression level and S-100βlevel was negatively correlated ( r =-0.939, P<0.01).Conclusion Propofol anesthesia has a protective effect on the brain tissues in the cerebral ischemia reperfusion rats by inducing Homer-1a mRNA expression, and this effect may be related to the decrease of S-100βlevel in the peripheral blood .