Influence of melatonin on PANC-1 cell proliferation and the mechanism
XIE Qiao
DONG Li-feng
XU You-qing
CUI Pei-lin
Abstract：Objective To observe the influence of melatonin on the PANC-1 cell proliferation and to investigate its mechanism .Methods PANC-1 cells were treated with different concentrations of melatonin , and MTT method was used to detect the proliferation of PANC-1 cells and to calculate the proliferation rate .Western blotting was used to detect the ex-pression of active fragment of Notch 1 ( NICD1) in PANC-1 cells.Results The proliferation rate of the control group 1 was 0.38%±0.03%, after adding the concentration of 1, 1 000 and 1 000 000 nmol/L melatonin, the proliferation rates of PANC-1 cells were 0.36%±0.02%, 0.32%±0.02%and 0.26%±0.01%, respectively.Significant difference was found between the control group 1 and the melatonin groups with concentrations of 1 000 and 1 000 000 nmol/L melatonin (all P<0.05).The proliferation rate of the control group 2 was 0.43%±0.02%, the proliferation rate in the DAPT group was 0.30%±0.02%(P<0.05).The expression of NICD1 in the control group 1 was 0.47 ±0.05.After adding the concentrations of 1, 1 000 and 1 000 000nmol/L melatonin, the expression of NICD1 was 0.32 ±0.04, 0.33 ±0.05 and 0.21 ±0.03, respectively.Significant difference in the NICD1 expression was found between the control group 1 and the melatonin groups with concentrations of 1 000 and 1 000 000 nmol/L melatonin (all P<0.05).The expression of NICD1 in the control group 2 was 0.63 ±0.05.The expression of NICD1 in the DAPT group was 0.38 ±0.03 (P<0.05).Conclusion Melatonin can inhibit the proliferation of PANC-1 cells, whose mechanism may be involved in regula-ting the Notch signaling pathway .
Keywords：melatoninNotch signaling pathwayPANC-1 cellsγ-secretase inhibitor
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )