Inhibitory effect of salvianolate on iron overload-derived oxidative stress injury of hematopoietic cells of umbilical cord blood
MU Juan
ZHAO Ming-feng
LI Yu-ming
ZHANG Yu-chen
CAO Xiao-li
LI Qing
Abstract：Objective To investigate the protective effect of salvianolate on the iron overload-derived oxidative stress injury of mononuclear cells of umbilical cord blood (UC-MNCs).Methods The UC-MNCs were divided into the control group, FAC group, low-dose salvianolate group, medium-dose salvianolate group, and high-dose salvianolate group.The control group received normal culture .The iron overload cell models in the FAC and salvianolate groups were established by adding 400 μmol/L FAC into the culture medium for 24 h on the second day.Then the low-dose, medium-dose and high-dose salvianolate groups were received 18, 50 and 160μg/mL salvianolate for 24 h, respectively on the third day .Then the levels of reactive oxygen species ( ROS) , apoptosis rate and LIP level were detected by flow cytometry , respectively .Re-sults The ROS level of the FAC group was higher than that of the control , the low-dose, medium-dose and high-dose salvianolate groups (all P<0.05).The apoptosis rate in the FAC group was higher than that of the control group and high-dose salvianolate group(P<0.05).The counts of colony-forming unit-erythroid (CFU-E), granulocyte-macrophage colo-ny-forming units ( CFU-GM) , erythropoietic burst-forming units ( BFU-E) and mixed progenitor cell colony-forming units ( CFU-mix) in the FAC group were significantly lower than those of the control group and high-dose salvianolate group ( P<0.05).Conclusions Salvianolate can reduce the oxidative stress caused by apoptosis and inhibition of proliferation and differentiation of hematopoietic cells .
Keywords：salvianolateiron overloadoxidative stressapoptosishematopoietic stem cellshematologic diseases
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )