Effect of PGD2 on TGF-β1/Smads signaling pathway in mouse lung fibroblasts
LIU Jian-ying
LIU Dai-shun
LIU Zhen-feng
HAN Bing
Abstract：Objective To investigate the effects of prostaglandin D2(PGD2) on TGF-β1/Smads signaling pathway in mice lung fibroblasts, and to provide a molecular study basis for airway remodeling of asthma.Methods The lung fibroblasts were randomly treated by different concentrations of PGD2 receptor inhibitor Laropiprant (0.3, 1, 3, 10, and 30μmol/mL) at differ-ent time (12 h, 24 h, 48 h, 72 h and 96 h).The inhibitory effect of Laropiprant on the cell growth was detected by MTT assay . The cells were divided into the control group, Laropiprant 0.3μmol/mL group, 1μmol/mL group, 3μmol/mL group, 10μmol/mL group and 30μmol/mL group.Each group was added PGD2 stimulant TGF-β2 (2.5 ng/mL) and then was cultured for 24 hours.The expression of TGF-β1, Smad3 and Smad4 was detected by PCR and Western blotting, respectively.Results After being treated with TGF-β2 (2.5 ng/mL) for 24 hours, the mRNA and protein expression of TGF-β1 , Smad3 and Smad4 showed a decreased tendency with the increased addition of Laropiprant concentration as compared with that of the control group (all P<0.05).The cell growth inhibition rate showed a increased tendency with the increased Laropiprant concentration and the pro-longed culture time.When Laropiprant concentration reached 1μmol/L, and the reaction time was 24 to 36 h, the cell growth in-hibition rate significantly improved.Conclusion PGD2 may cause the airway remodeling by regulating TGF-β1/Smads signaling pathway in L-929 mouse lung fibroblasts.
Keywords：lung fibroblastsbronchial asthmaprostaglandin D 2LaropiprantTGF-β1/Smadsmice
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )