Effects of metformin combined with oxaliplatin on VEGF-C, VEGFR-3, CD44v6 andβ-catenin expression of lung cancer xenograft in nude mice
CHEN Yu-qin
CHEN Gang
Abstract：Objective To investigate the effects of metformin combined with oxaliplatin on the expression levels of vascular endothelial growth factor-C (VEGF-C), vascular endothelial growth factor receptor-3 (VEGFR-3), CD44v6 andβ-catenin of lung cancer xenograft in nude mice .Methods We established human lung cancer xenograft models in the nude mice.The nude mice were randomly divided into metformin-treated, oxaliplatin-treated, metformin combined with ox-aliplatin-treated and untreated control groups .Forty-two days after administration , all the nude mice were sacrificed and tumor tissues were sampled.The protein and mRNA expression of VEGF-C, VEGFR-3, CD44v6 and β-catenin in the tumor tissues was detected by immumohistochemical method and florescent real-time quantitative PCR , repectively .Results The respective tumor inhibition rates of metformin-treated group , oxaliplatin-treated group and metformin combined with oxaliplatin-treated group were 28.97%, 34.37%and 50.27%.The expression levels of VEGF-C,VEGFR-3, CD44v6 andβ-catenin protein and mRNA in the oxaliplatin-treated and metformin combined with oxaliplatin-treated groups were lower than those in the control group, respectively (all P<0.05).Compared with the metformin-treated group and oxaliplatin-treated group, the expression levels of VEGF-C,VEGFR-3, CD44v6 and β-catenin protein and mRNA of the metformin combined with oxaliplatin-treated group were lower, respectively (all P<0.05).F values about comparison of VEGF-C, VEGFR-3, CD44v6 andβ-catenin proteins within four groups were 79.281, 151.275, 171.294 and 164.149, respectively (all P<0.01);F values about comparison of VEGF-C,VEGFR-3, CD44v6 andβ-catenin mRNA within four groups were 78.897, 81.029, 64.100 and 83.892, respectively (all P<0.01).Conclusions Using oxaliplatin or metformin can in-hibit the expression of VEGF-C,VEGFR-3, CD44v6 and β-catenin.The combination of metformin and oxaliplatin can en-hance the anti-tumor efficacy .
Keywords：lung neoplasmsmetforminoxaliplatinvascular endothelial growth factor-Cvascular endothelial growth factor receptor-3CD44 variant protein 6β-catenin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )