Effects of resveratrol on apoptosis of βcells exposed to palmitic acid in vitro
XU Bi-lin
SHEN Tian
YU Ming
WANG Hong-ping
ZHANG Zhi-jian
ZHU Jin-yue
Abstract：Objective To evaluate the effects of resveratrol ( Res) on the apoptosis of βcells exposed to palmitic acid (PA, a free fatty acid).Methods βcells in the period of logarithmic phase were randomly divided into A-F groups.A group was blank group excluding PA , and B group was cultured with 500 μmol/L PA.C-F groups were put in culture me-dium with 0.1, 1, 10, 50 μmol/L Res+500 μmol/L PA respectively.After 24 hours, flow cytometry was used to calcu-late the survival rate and the apoptosis rate of βcells.Results The survival rate of A-F groups were 90.31%±4.70%, 5.07%±2.27%, 8.37%±2.06%, 21.99%±2.47%, 88.67%±1.17%, 5.20%±1.72%, while the apoptosis rates were 7.40%±2.99%, 95.82%±1.94%, 83.36%±2.06%, 68.94%±2.19%, 11.03%±1.08%, 95.65%±1.72%, respectively.There were significant differences among A group and B-F groups (all P<0.05).Compared with B group, C-E group displayed remarkably lower apoptosis rates and higher survival rates (all P<0.01).There were signif-icant differences among C group and D , E groups (all P<0.01); and compared with D group, E group displayed lower apoptosis rate and higher survival rate (all P<0.01).Conclusion PA increases βcell apoptosis in vitro, Res at some appropriate concentrations may reduce βcell apoptosis induced by PA .
Keywords：diabetesislet cellsapoptosisresveratrolpalmitic acidflow cytometry
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 11-13 )