Discussion of the optimum conditions and parameters of separation and purification of total flavones from Malus doumeri (Bois) Chev by macroreticular resin
CHEN Hong-tao
LIU Yuan-huan
QIN Xue-qian
CAI Dan-zhao
Abstract：Objective To explore the optimum technological parameters of the separation and purification process of total flavones from Malus doumeri (Bois) Chev with macroreticular resins.Methods With the static and dynamic saturat-ed adsorption and elution rate as index , compare the advantages of nonpolar resin D 101 , weak polarity resin AB-8, medium polarity resin HPD-450 used in isolation and purification of total flavones from Malus doumeri (Bois) Chev, and optimize the adsorption process parameters of selected optimum resin by investigating the total flavones recovery rate .Results The static saturated adsorption of D 101 , AB-8, HPD-450 were 94.802, 118.494, 98.317 mg/g dry resin, the static elution rate were 83.434%, 91.253%and 83.434%respectively;the amount of total flavones eluted were 91.490, 115.567, 98.528 mg, elution rate were 85.852%, 93.217%and 89.051%respectively.Eluted with five times resin volume of 70%etha-nol, flow rate of 1.5 mL/min, the recovery rate of total flavones was 94.09%, the purity of which was 73.77%.Conclu-sion The comprehensive performance of AB-8 resin was the best , the optimum process parameters were that of eluted with five times resin volume of 70%ethanol and 1.5 mL/min flow rate.
Keywords：total flavones of Malus doumeri ( Bois) Chevmacroreticular resinseparation and purification
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 7-10 )