Effect of chlorogenic acid on Th17/Treg shift in deep vein thrombasis rats

SUN Tao; LI Xia; WANG Bin

doi:10.3969/j.issn.1002-266X.2015.08.002

Abstract：Objective To evaluate the role of Th17/Treg shift in deep vein thrombasis ( DVT) rats and elucidate the mechanism of chlorogenic acid to treat DVT. Methods 60 rats were divided randomly into six groups (10 rats in each group). A, B, C, D groups according to the Reyers method to establish the model of DVT, give chlorogenic acid respec-tively 40, 20, 10 mg/kg and the amount of normal saline lavage in succession for 7 days.10 rats with sham operation were taken as E group.F group no processing. The proportions of Th17 subset and Treg subset were detected by flow cytometry. The protein expressions of IL-17, TGF-βin serum were analyzed by ELISA assay, and to analyze the correlation of the pro-portion of Th17/Treg with serum levels of inflammatory markers. Results Compared with E and F groups, the proportion of Th17 subset increased while that of Treg subset decreased, the protein expression of IL-17 enhanced while that of TGF-βinhibited markedly in B-D groups (all P<0.05).Compared with D group, the proportion of Th17 subset was down-reg-ulated while that of Treg subset was up-regulated significantly A-C groups (all P<0.05), the protein expression of IL-17 decreased while that of TGF-βincreased obviously in A-C groups (all P<0.05).The values of Th17/Treg and IL-17 were positively correlated, and negatively correlated with TGF-β, all P<0.05.Conclusions Th17/Treg shift was involved in DVT.Chlorogenic acid down-regulated the Th17 subsets and up-regulated the Treg subsets;thereby, chlorogenic acid exer-ted the curative effect on DVT rats.