Protective effect of ginsenoside on hematopoietic progenitor cells under ionizing radiation
HUANG Ying
LIANG Xiao-yan
LI Pin-jin
HU Jun
KANG Xiao-li
Abstract：Objective To investigate the protective effect of ginsenoside on hematopoietic progenitor cells ( HPCs ) under different intensity of ionizing radiations .Methods The HPCs were divided into the control group and the observe group, then to add ginsenoside into the observe group and cultivated for 24 hours.Respectively exposed under X-ray irradi-ations on 1, 2, 5 Gy.Cell viability was studied by MTT method .The generation of reactive oxygen species ( ROS) after 24 hours, the expression of caspase-3 mRNA and Nrf-2 mRNA were measured by means of flow cytometry and Western blot , respectively .Results With the increase of radiation dose , cell viability reduced , the generation of ROS increased , the ex-pression of caspase-3 mRNA enhanced and the Nrf-2 mRNA decreased, gradually, all P<0.05.Compared with control group with the same radiation dose , cell viability in the observe group increased , the generation of ROS and the expression of caspase-3 mRNA decreased, the Nrf-2 mRNA increased, all P<0.05.Conclusion Ginsenoside could protect HPCs from radiation effects , which was correlated with anti-apoptosis and inhibition of oxidation stress .
Keywords：ginsenosidehematopoietic progenitor cellsionizing radiationreactive oxygen speciescysteine protein-aseNrf-2
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )