Establishment of gelatin-chitosan composite neurotrophins-3 nerve scaffold and its biocompatibility
QIN Li-na
Abstract：Objective To fabricate the gelatin-chitosan composite neurotrophins-3 nerve scaffolds and to determine its biocompatibility .Methods We mixed the gelatin and chitosan solutions , then added a trace of neurotrophin-3.After mak-ing the injection mold , we fabricated the nerve guidance scaffolds by the freeze-drying technique .Then the characteristics of the scaffold were observed by scanning electron microscope morphology , and the scaffold diameter , porosity, etc were de-tected.We extracted the gelatin chitosan composite neurotrophin-3 nerve scaffold material extracts , and then we observed the effects on the growth and differentiation of neural stem cells;the electrophysiological properties of the differentiated cells were observed by the patch-clamp technique .Results The inside diameter of gelatin-chitosan composite neurotrophins-3 nerve scaffold was (267.0 ±13.8) μm and porosity was 90.0%.Scanning electron microscopy showed that neural stem cells grew well on the scaffold.Under the induction of retinoic acid , neural stem cells differentiated into neuron-like cells, and showed structural of synaptic connections between neurons initially .What′s more, the cells differentiated were detected the neuron-like cell electrophysiological properties by the patch-clamp technique .Conclusion The gelatin-chitosan com-posite neurotrophin-3 nerve scaffolds were successfully constructed , and had good biocompatibility with neural stem cells .
Keywords：spinal cord injuriesscaffold materialsneurotrophin-3retinoic acidneural stem cells
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )