Effect of miRNA-137 transfection on human uveal melanoma cell line invasion
CHEN Jun-jie
ZHENG Na-feng
YE Zi-ying
LIN Jun-shan
OU Rui-zhang
ZENG Yu-ting
Abstract：Objective To investigate the effects of miR-137 gene transfection on human uveal malignant melanoma cell lines M23.Methods miR-137 mimics were transfected to M23 by Lipofectamine package Lipofectamine TM 2000.Cell invasion was detected by transwell invasion assay 18 h after transfection .MMP-2 and MMP-9 proteins expression were in-vestigated by Western blot and ELISA .Results Transwell invasion assay showed that the number of M 23 cells in miR-137 mimics group was significantly lower than negative control group .Western blot and ELISA confirmed that miR-137 mimics could significantly inhibit the expressions of MMP-2/-9 protein and secreting .Conclusion miR-137 gene transfection can inhibit the invaion of human uveal malignant melanoma cell by reduce the expression of MMP -2 and MMP-9.
Keywords：uveal melanomainvasionmiR-137matrix metalloproteinase
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 18-20 )