Antifungal susceptibility and genotyping analysis of aspergillus fumigatus caused lower respiratory tract infection
Abstract：Objective To study the resistance and homology of aspergillus fumigatus that caused lower respiratory tract infection.Methods The 31 clinical strains of aspergillus fumigatus were respectively isolated from the sputum and bronchoalveolar lavage fluid of patients who suffered from lower respiratory tract aspergillus fumigatus infection .The sus-ceptibility of five antifungal agents against aspergillus fumigatus was measured by liquid microdilution method and the ho -mology of strains was analyzed with random amplified polymorphic DNA (RAPD)assay.Results The resistance of the 31 tested aspergillus fumigatus strains was 3.2%to voriconazole,12.9%to itraconazole,25.8%to amphotericin B whereas all sensitive to caspofungin and micafungin.By RAPD,the 31 strains were divided into 10 genotypes(A~J),among which Type B was the major prevalent genotype that consisted of 10 strains.Conclusions The genotype B was proved to be the relevant type that caused lower respiratory tract aspergillus fumigatus infection in the surveyed hospital .Voriconazole ,caspo-fungin,micafungin exhibited favorable antifungal activity against Aspergillus fumigatus isolates in vitro .
Keywords：aspergillus fumigatusdrug resistancegenotyping
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 14-17 )