Protective effect of hydrogen saline in oxidative stress insulted enterocytes
MU Huai-bo
SHENG Qing-feng
WU Wei
LV Zhi-bao
Abstract：Objective To explore the effect of hydrogen saline in oxidative stress insulted enterocytes .Methods Caco-2 and IEC-6 cells were divided as 3 groups:control, model and hydrogen treated groups .Cells in control group was treated with normal RPMI1640 medium.Model group was treated with RPMI1640 medium containing antimycin A .Cells in hydrogen treated groups was treated with RPMI 1640 medium containing antimycin A and H 2.①Levels of O2-· 、H2 O2 adn · OH;②Mitochondrial membrane potential;③MDA and 8-OH-G;④Cell apoptoiss and LDH activity;⑤Proinflammatory factors(IL-6、TNF-α、IL-1β)、anti-inflammatory factors(IL-10),expressions of Nrf2 and the downstream target genes (HO-1、SOD、Cat、GPx);⑥Expression of phosphorylation-Nrf2 were detected for further study .Results We found that H2 ef-fectively reduce the reactive oxygen specis , cell apoptosis and activity of LDH in culture medium and improved the mito-chondrial membrane potential .H2 activated the phosphorylation of Nrf 2.H2 significantly reduced the release of inflamma-tory cytokines and promoted the downstream genes expressions of Nrf 2.Conclusions Our work demonstrated that H2 showed favorable ability in necrotizing enterocolitis treatment .The mechanism was be closely related with Nrf 2-ARE signa-ling.
Keywords：necrotizing enterocolitishuman colon adenocarcinomahydrogenNrf2-ARE signaling pathwayoxida-tive stress
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-7 )