Protective effects of hydrogen saturated saline alleviating myocardial ischemia reperfusion injury in rats

LIU Xue-cong; LIU Fu-lin; LI Zhi-lin; ZHOU Xiao-dong; SHEN Wen-zeng; ZHOU Cheng

doi:10.3969/j.issn.1002-266X.2015.06.001

Abstract：Objective To explore the protective effects of hydrogen saturated saline in induced myocardial ischemia reperfusion injury in rats .Methods Forty-eight rats were divided randomly into the experimental group (24) and the con-trol group (24).The rats of experimental group were treated with K-R solution combined with hydrogen saturated saline , the controls were given K-R solution only .After the treatment , the hearts were excised rats and Langendorff model .And the heart of the in vitro by inverse perfusion for 10 minutes, normal temperature place for 20 minutes, 20 minutes of reper-fusion.The solution of the cardiac muscle tissue of the rats were treated with saline and then the accounts of malondialde -hyde (MDA)and the activity of superoxide dismutase (SOD) , and the changes of left ventricular end-diastolic pressure ( LVEDP) were determined to compare the different protective effects .Results The accounts of MDA in the control group were the highest , there was a significant difference between the experimental group and the controls concerning with the re -sults of ischemia period and reperfusion period (P<0.01, P<0.05).There were differences among the ischemia period and reperfusion period and preischemia period in the control group (P<0.05).In the experimental group, there was not significant difference among the ischemia period and reperfusion period and the preischemia period .There was a significant difference between the experimental group and the controls considering the activity of SOD (P<0.01).The accounts of LVEDP of the control group was obviously high (P<0.05).The level of LVEDP in experimental group was obviously lower than in the control group (P<0.05),but had no significant difference to ischemia period .Conclusion Hydrogen rich sa-line is quite effective in alleviating myocardial ischemia reperfusion injury .