Protection of quercetin against liver ischemia reperfusion injury in rats

WANG Jia-jia; ZHONG Jia-ling; ZHANG Juan; HUANG Wen-fang

doi:10.3969/j.issn.1002-266X.2015.05.008

Abstract：Objective To establish rat ischemia reperfusion injury model and evaluate the protection effect of querce -tin against liver ischemia reperfusion injury in rats .Methods A total of 80 healthy rats were randomly divided into control group(n=5), sham group(n=25), ischemia reperfusion injury group(n=25) and quercetin group(n=25).The later three groups were divided into 0, 6, 12, 24, 72 h subgroups(n=5), respectively.At the end of the experiment, the blood samples and liver tissues were collected .The levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αwere determined by automatic biochemistry analyzer and competitive inhibition ELISA , respectively .The morphology change of liver were de-tected by HE staining, and the apoptosis of liver cells were detected by TUNEL .Results In 0, 6, 12, 24, 72 h sub-group, the levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αincreased, especially in 12 h group, which had significant statistically difference compared with control or sham group (all P<0.05).Under light microscope, there was no signifi-cant change in control or sham group .In ischemia reperfusion injury group , liver cells degenerated significantly , liver cord structure disappeared, some liver cells necrosed.However, in quercetin group, only part liver cells degenerated , part liver cord structure exsited , which was consistent with the results of apoptosis index .Conclusion Quercetin is beneficial for liver ischemia reperfusion injury .