Assessment of liver reserve function based on determination of phenacetin and paracetamol by spectrophotometry
LU Su-mei
REN Rui
SUN Tao
MA Yong-mei
MA Wan-shan
Abstract：Objective To establish a liver reserve function assessment method based on detection of phenacetin and paracetamol by spectrophotometry .Methods To screen the spectrophotometric color system , determine the maximum ab-sorption wavelength , analyze the influence of color system in different factors , optimize optimal conditions that phenacetin and paracetamol hydrolyze , and then verify the application of this technology about the established system .Results The technology system detected phenacetin and paracetamol of blood samples using spectrophotometry was established , in which 3 mol/L hydrochloric acid was added to samples to hydrolyze for 30 min, then added 0.02%1,2-naphthoquinone-4-sulfon-ate, 1%sixteen alkyl bromide and 2%sodium hydroxide (or 3%sodium carbonate) (ratio of 1∶6∶1∶2 or 3), and then the absorbance was measured under 500 nm and 570 nm to calculate each concentration .Resolution and repeatability of technology used for determining phenacetin and paracetamol of plasma samples were comparable with HPLC .Conclusion Ratio of phenacetin and paracetamol under established spectrophotometric system could assess liver reserve function .
Keywords：liver reserve functionspectrophotometryphenacetinparacetamol
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 14-17 )