Construction semi-random Adnectin ribosome display library by PCR amplification and gene synthesis
GUO Yong-hong
WEN Jiao
WANG Jun-hong
Abstract：Objective To construct semi-random Adnectin ribosome display library .Methods Adnectin ribosome display library was constructed by PCR amplification combining with gene synthesis method .PCR products contained the restrictive sites of SpeⅠbased on nonsense mutation .The recombinant plasmids were identified by restriction endonuclease enzyme analysis and DNA sequence .The positive recombinant clones were screened and library capacity was measured by blue-white test.Result The library was confirmed by sequencing and library capacity was 1.54 ×1013/mL.Conclusions To corstruct successful Adnectin semi-random ribosomes display library ( mRNA) used simple and easy method .The built library laid the foundation for Adnectin affinity with various related protein .
Keywords：AdnectinPCR amplificationgene synthesisribosomes display library
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-7 )