Expression of neurokinin-1 receptor and it′s effect in different stages of young asthmatic rats of airway remolding

WEI Bing; SHANG Yun-xiao; ZHANG Chao; LIU Ya-li; MA Ming

doi:10.3969/j.issn.1002-266X.2015.02.006

Abstract：Objective To investigate the expression of neurokinin-1 receptor and it′s effect in different stages of young asthmatic rats of airway remolding.Methods A total of 64 young SD rats were randomly divided into asthma group and normal control group with 32 rats in each group.Asthma group were challenged with OVA to establish asthma rat model .The control group underwent treatment with normal saline instead of OVA .Each group was randomly divided into 4 subgroups and they were anatomized at 2 weeks, 4 weeks, 6 weeks, 8 weeks.HE staining and image analysis were used to measure Wat/Pbm and Wam/Pbm.Alkaline hydrolysis method was used to determine the hydroxyproline content .The expression of neurokinin-1 receptor were respectively located and measured by immunohistochemistry and Western blotting method.The level of neurokinin-1 receptor mR-NA were detected by using Real-time PCR.Results HE staining of control group showed that bronchial epithelial integrity , in-flammatory cells infiltrated rarely and smooth muscle layer thinner.HE staining of asthma group showed that pathological changed obviously, especially in 8w group.Wam/Pbm and hydroxyproline of airway smooth muscle in asthma group were both higher than the control group(P<0.05).Wat/Pbm of 4 weeks, 6 weeks, 8 weeks in sthma group were higher than the control group (P<0.05).The comparion of Wat/Pbm,Wam/Pbm and hydroxyproline of asthma subgroups were 8 weeks>6 weeks>4 weeks>2 weeks (P<0.05).The expression of neurokinin-1 receptor mRNA and protein in asthma groups were higher than the control groups and 8 weeks>6 weeks>4 weeks>2 weeks (P<0.05).Conclusions Asthma rats appear airway remodeling in a early phase of incidence.The expression of neurokinin-1 receptor mRNA and protein increased in bronchial tissue with the time of air-way remodeling.Sensory neuropeptide receptors play an important role in the development of asthmatic airway remodeling .