Effect of matrine on the expression of B7-H1 in tubular epithelial cells
WU Yuan
Abstract：Objective To explore the effect of matrine on expression of B 7-H1 in tubular epithelial cells .Methods①The effect on different dose of matrine:human HK-2 cells were cultured under five culture conditions to observe and di-vided into 4 groups, which was control group(5%fetal calf serum+DMEM for 24 h), model group (IFN-100 g/L for 24 h), matrine group (IFN-100 g/L+0.8 g/L matrine for 12 h,16 h and 24 h) .The expression of B7-H1 mRNA was evalu-ated by using real time RT-PCR.The surface expression of B7-H1 on HK-2 was analyzed by using flow cytometry .②The effect on different time of matrine: cell were divided into control group (5% fetal calf serum+DMEM for 24 h), model group (IFN-100 g/L for 24 h), matrine group (IFN-100 g/L+0.8 g/L matrine for 12 h,16 h and 24 h).The expression of B7-H1 mRNA was evaluated by using real time RT-PCR.Results The expression of B7-H1 on HK-2 cells was de-creased in normal condition .IFN-can significantly increased the expression of B 7-H1.Matrine decreased the B7-H1 ex-pression of HK-2 cells in a dose and time dependent way at mRNA levels and in a dose dependent way at protein levels ( P<0.05).Conclusion B7-H1 mRNA and protein increased under the condition of inflammation induced by IFN -γ.Ma-trine could down regulate the expression of B 7-H1 mRNA and protein .
Keywords：matirnetubular epithelial cellB7-H1co-stimulatory molecule
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )