Expression of herg gene, protein and changes of HERG current in gastric cancer cells
SHAO Xiao-dong
ZHANG Yong-guo
CHEN Jiang
WANG Jin-ling
GUO Xiao-zhong
REN Li-nan
Abstract：Objective To investigate the expression of herg gene , HERG protein and HERG current in gastric cancer cells.Methods Gastric cancer cells and gastric epithelial ( GES) cells were cultured to logarithmic phase .The expres-sions of herg mRNA and HERG protein in gastric cancer cells and GES cells were measured by using RT -PCR and Western blot, respectively.The whole cell configuration of the patch-clamp technique was employed to record HERG currents in va-rious cells .Results HERG mRNA and protein were positively expressed in four gastric cancer cell lines .Expression level of HERG protein in AGS cells was lower compared with the other three gastric cancer cell lines (P<0.05).There was negative expression of herg mRNA and HERG protein in GES cell line .HERG current was detected in gastric cancer cell , whereas there was no HERG current in GES cell .Conclusion Herg gene and HERG protein were highly expressed in gas-tric cancer cells and HERG current was exclusively detected in gastric cancer cells .HERG protein was associated with car-cinogenesis of gastric cancer and may serve as a diagnostic marker for gastric cancer .
Keywords：stomach neoplasmshuman ether-a-go-go-related geneHERG proteinHERG currentdelayed rectifier potassium channelneoplastic processes
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )