EstabIishment of PD modeIs in SD rats induced by rotenone
CHAI Xing-xing
BAO Bo
YE Cheng
LI Hui-hua
ZHU Shao-ping
ZENG Min-juan
Abstract：Objective To established PD models by long term subcutaneous injection of rotenone .Methods Totally 36 SD rats were randomly divided into two groups:control group contained 12 rats and model building group contained 24 rats.Rats in model building group were treated with rotenone at dose of 1.5 mg/(kg? d) by subcutaneous injection for 30 days, withdrawal one day a week .Rats in control group were treated with the same volume of sunflower oil .After the end of subcutaneous injection , the behavior changes of SD rats were tested and then sacrificing the rats to take out the brain tis -sues, detecting the expression of TH-positive cells in substantia nigra by counting out the number of TH-positive cells.Re-sults The behavior changes of SD rats in control group were not obvious , but the number of dopaminergic neurons in-creased.The behavior changes of SD rats in model building group was obvious , and their dopaminergic neurons numbers decreased .SD rats in model building group met the diagnostic criteria of PD .Conclusions The methods of long-term sub-cutaneous injection of rotenone could establish PD models in SD rats .
Keywords：Parkinson diseaserotenoneratsTH-positive cells
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-11,14 )