Effect of human cartiIage gIycoprotein-39 on proIiferation of precartiIaginous stem ceIIs of aduIt articuIear cartiIage
CHEN Yue-ping
DONG Pan-feng
ZHANG Xiao-yun
YUAN Zhen-zhong
RAO Yi
Abstract：Objective To investigate the effect of HCgp-39 on adult articular cartilage precartilagincus stem cells proliferation.Methods HCgp-39(0, 25, 50, 100, 500 ng/mL) were added into precartilaginous stem cells .After 0, 24, 48, 72 h, stem cells were collected, and subjected to 10μL MTS, and then detecting OD490, comparing the cell sen-sibility to HCgp-39 in all groups.Results OA group:After 24 hours, the mean and standard deviation of cells subjected to 25, 50, 100, 500 ng/mL HCgp-39 were 0.81 ±0.01, 0.88 ±0.02, 0.93 ±0.01, 1.03 ±0.04, separately; after 72 hours, the mean and standard deviation were 1.02 ±0.05, 1.12 ±0.01, 1.21 ±0.01, 1.28 ±0.01, separately.When Compared with stem cells without subjecting to HCgp-39 , there was no statistically differences; normal group: after 24 hours, the mean and standard deviation were 0.67 ±0.01, 0.63 ±0.01, 0.61 ±0.01, 0.58 ±0.01;after 72 hours, the mean and standard deviation were 1.18 ±0.02, 1.1 ±0, 1.08 ±0.01, 0.97 ±0.02;comparing with cells without subjec-ting to HCgp-39, the differences were statistically significant .Conclusions There are articular cartilage cell proliferation in the adult articular cartilage;HCgp-39 had the promotional effects on proliferation of precartilaginous stem cells .
Keywords：osteoarthritisprecartilaginous stem cellshuman cartilage glycoprotein-39proliferation of cells
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )