Expression and clinical significance of miR-181a, miR-200a, miR-200c in lung cancer tissues and cells

doi:10.3969/j.issn.1002-266X.2014.48.07

Abstract：Objective To observe the expression and significance of miR-181a, miR-200a, miR-200c in lung cancer tissues and cells.Methods RT-PCR method was used to detect the expression of miR-181a, miR-200a, miR-200c in lung cancer cell lines NCI-H1299, NCI-H358, H460, A549 and normal human bronchial epithelial cell line HBE , and in the lung cancer tissues and adjacent tissues .To analyze the relationship between the expression of miR-181a, miR-200a, miR-200c and clinicopathological parameters of lung cancer by means of Mann-Whitney.Results miR-181a expression in NCI-H1299, NCI-H358 and A549 were lower than that in HBE (all P<0.05).miR-200a expression in NCI-H1299, NCI-H358, H460 and A549 were lower than that in HBE (all P<0.05).miR-200c expression in NCI-H358 and H460 were higher than that in HBE (all P<0.05).The expression of miR-181a and miR-200a were no significant differences between lung cancer tissues and cancer-adjacent tissues (all P>0.05).miR-200c expression in lung cancer tissues was higher than that in cancer-adjacent tissues (P<0.01).miR-200a expression in the lung cancer patients with lymphatic metastasis was lower than that in lung cancer patients without lymphatic metastasis (P<0.01).Conclusions The expression of miR-181a, miR-200a were generally decreased , while miR-200c was generally increased in lung cancer cell lines and tissues , which maight be related to the occur of lung cancer .