Effects and mechanisms of Mask Dark Dragon Pelle on diabetes mellitus rabbits fracture healing
LIANG Shan
HE Pan
WANG Yu-si
Abstract：Objective To observe the effects and mechanisms of Mask Dark Dragon Pelle on diabetes mellitus ( DM) rabbits fracture healing.Methods A totle of 36 rabbits were divided into A, B, C groups, 12 rabbits in each group.B and C groups were DM models , A group was normal control .Then fracture of the right tibia and fibula were builted in three groups.The rabbits in C group were received intragastric administration of 1.0 g/kg Mask Dark Dragon Pelle +5 mL nor-mal saline, the equal volume of normal saline were given in A and B groups .At 1, 2, 4 weeks after treatment, four rabbits in each group were taken to study .The bone mineral density ( BMD ) was detected by dual-energy X-ray absorptiometry ( DEXA);and the callus were collected for HE staining to detect pathological changes ;the level of NGF in callus was test-ed by Western blot .Results BMD in B group showed an downward trend during the process of fracture healing , the BMD in B group decreased than A and C groups at 1, 2, 4 weeks (P均<0.05).Among three groups, group C displayed the best callus formation in pathological changes , and fracture healing was significantly better than that in the other two groups . At the same time, the expression of NGF was significantly higher in group C than that in group B ( P<0.05).Conclu-sions The expression of NGF decreased in callus of DM rabbits .Mask Dark Dragon Pelle accelerates the DM fracture healing, its mechanism maybe related to NGF expression up-regulation.
Keywords：fracturediabetes mellitusMask Dark Dragon Pellenerve growth factorrabbit
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 13-15 )