Effects and mechanisms of Qi-boosting toxin-resolving formula on the precancerous lesions of nasopharyngeal epithelia in TgN (p53mt-LMP1)/HT transgenic mice

doi:10.3969/j.issn.1002-266X.2014.48.04

Abstract：Objective To investigate the intervening effect and the mechanism of Qi-boosting toxin-resolving formula (QBTRF) on the precancerous lesions of nasopharyngeal epithelia in TgN (p53mt-LMP1)/HT transgenic mice.Methods 72 transgenic mice of TgN (p53mt-LMP1)/HT were randomly divided into A, B, C groups, 24 mice in each group.24 healthy wild mice were as group D .N,N′-dinitrosopiperazine ( DNP) was given to all the mice by subcutaneous injection . At the same time, the mice in group A, B, C were treated by normal saline, QBTRF, tretinoin by lavage respectively;group D without any additional treatment;course of treatment was 14 weeks.When the treatment was over , all mice were put into death to collect tissue samples from their nasopharyngeal mucous membrane .The atypical hyperplasia was observed by optical microscope;the ultrastructural pathology was observed by electron microscope;Cx43, Cx32, Cx26 proteins and their responsible mRNA were measured by immunohistochemical staining , Western blot and RT-PCR.Results In optical microscope , the nasopharyngeal epithelia of group A showed obvious atypical hyperplasia , which had the characteristics of cancerization;the part of nasopharyngeal epithelia in B and C groups showed atypical hyperplasia ;hyperplasia in D group . Under the electro microscopy , the intercellular space increased , cell junction length reduced , cell junction number de-creased in group A;B,C and D had no above-mentioned changes .The expression levels of Cx43, Cx32, Cx26 proteins and mRNA in B and D groups were significantly higher than those in group A and C (all P<0.05).Conclusions QBTRF had the role of intervening the development of precancerous lesion in nasopharyngeal epithelia of TgN (p53mt-LMP1)/HT, which may be correlated with the gap junctional intercellular communication recovering .