The observation on application of growth factor two-step method for inducing adipose stem cells into nerve cells in rat
ZHOU Liu-jie
XIA Ying-peng
Abstract：Objective To investigate the effect of rat adipose tissue-derived cells ( ADSCs) into neural cells with a growth factor two-step induction method .Methods ADSCs were isolated from the fat pads in inguinal and retroperitoneal of Sprague Dawley rats , and the third generation cells were cultured .First, the cells were induced with DMEM/F12+2%B27+20 ng/mL EGF+20 ng/mL bFGF for 7 days, and then DMEM/F12+1μmol/L RA for another 7 days.Immunocyto-chemistry was taked to identify the expression of the neuron specific markers Nestin , MAP2, NeuN and GFAP.Results ADSCs were reaped from rat fat tissue , and could passage in a considerable large amount .ADSCs could strongly express CD73(99.17%), CD90(98.61%), CD105(96.20%)and lowy expressed CD34(0.36%).The ADSCs induced for 7 days aggregated as floating cell spheres and positive for Nestin , then strongly express MAP2 and NeuN, and lowy expressed GFAP after 14 days.Conclusion The growth factor two-step induction method can efficiently induce ADSCs to differenti-ate into nerve cells .
Keywords：growth factoradipose stem cellsnerve cells
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-20 )