Effect of gypenosides on the proliferation of HMCs and expressions of TGF-β1 and FN mRNA induced by AGEs
ZHOU Kang
TANG Ling
XIAO Fu-ying
WANG Yan
Abstract：Objective To investigate the effect of gypenosides ( GP) on the proliferation of human mesangial cells ( HMCs) and the expression of TGF-β1 and FN mRNA induced by AGEs .Methods Logarithmic growth phase HMCs were divided into A, B, C, D groups, which were induced by AGEs (200 mg/L) in A, B, C groups, group B was inter-vened by different doses of GP at the same time , while group C was given 0.1 mmol/L aminoguanidine hydrochloride , group D was not given any induction and intervention .The inhibition of HMCs proliferation was determined by MTT assay . The expressions of TGF-β1 and FN mRNA in HMCs in all groups were detected by semi-quantitative RT-PCR.Result Compared with group D and group A , the inhibition of cell proliferation in Group B increased in a dose-and time-dependent manner (all P<0.01).Compared with group C,the inhibition of cell proliferation in group B decreased intervened by 25, 50, 75, 100 mg/L GP, while increased by 150, 200 mg/L GP, and in a dose and time dependent manner (all P<0.01). Compared with group D , group B increased in a dose-dependent manner ( all P<0.01);Ccompared with group A , the ex-pression of TGF-β1,FN mRNA in group B decreased in a dose-dependent manner(all P<0.01).Conclusion GP can in-hibit the over proliferation of HMCs induced by AGEs , the mechanism may be related to down-regulate the over-expressions of TGF-β1 and FN mRNA.
Keywords：gypenosidesdiabetic nephropathyadvenced glycation end productshuman mesangial cells
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )