Effects of bufalin and cisplatin on the proliferation and apoptosis of tongue squamous cell line Tca8113

LV Yin-peng; ZHOU Hui; SONG Jia-xin

doi:10.3969/j.issn.1002-266X.2014.46.006

Abstract：Objective To investigate the effects of bufalin and cisplatin on proliferation and apoptosis of tongue squa -mous cell line Tca8113.Methods Tongue squamous cell line Tca8113 was cultured and the logarithmic phase cells were divided into control, bufalin(10,20,40,80,160 nmol/L), cisplatin(2.5,5,10,20,40 μg/mL) and combination groups. MTT assay was used to examine the inhibition rate of cell growth when cell were treated at various concentrations of bufalin and cisplatin alone or in combination and calculate inhibiting concentration of 50%cell growth ( IC50 ) .The corresponding concentrations of three drug groups were selected based on IC 50 of Bufalin and cisplatin at 48 h.Apoptosis was evaluated by flow cytometry.The protion expression of Bax ,Bcl-2,Caspase-3,P-ERK and Erk were examined by Western blot method . Results Bufalin or cisplatin effectively inhibited the growth of Tca 8113 cells in a concentration-dependent manner at cer-tain range of concentrations .The inhibitory rate of combined treatment with bufalin and cisplatin was significantly higher than that of bufalin or cisplatin alone (P<0.05).The combination index(CI) values were all <1.When the Tca8113 cells were exposed to bufalin (40 nmol/L) combined with cisplatin (10 μg/mL) for 24 h, apoptosis rates were significantly in-creased.The difference was significant between drug treatment groups and control group (P<0.05).Both of bufalin and cisplatin could decrease the protein expression of p-Erk and increase the protein expression of Bax and Caspase-3.All these effects were enhanced when identical experiments were treatment with combination of the two drugs .Bufalin or cisplatin a-lone did not affect the protein expression of Bcl-2 obviously , but it was significantly decreased when combination used ( P<0.05).Conclusions Bufalin combined with cisplatin has synergistic effects in inhibiting the growth and inducing apopto -sis of Tca8113 cells and regulating the proteins expression of apoptosis-related and inhibiting Erk signaling pathway may be the related mechanism .