Effects of z-VAD-fmk on apoptosis in rats kidneys after scald
LI Hong-wei
LIU Qun
ZHU Bao-chang
SHENG Xiao-hui
Abstract：Objective To observe effects of z-VAD-fmk on apoptosis in rats kidneys after scald .Methods Forty-eight rats were randomly assigned into control group (n=24) and treatment group(n=24).The two groups were made the severe scald model For the treatment group , z-VAD-fmk were administrated immediately at the doze of 3 mg/kg by caudal vein injection and 1.5mg/kg, per 12 h by intraperitoneal injection .The control group was given 0.9%saline instead of z-VAD-fmk with the same method .The two groups were injected sodium lactate ringer ’ s injection after scald according to the formula of Parkland and sodium lactate ringer ’ s injection was reduced correspondingly .Six rats were killed at 2 h, 8 h, 24 h, 48 h in each group and the kidneys were immediately removed respectively at each time point mentioned above .The de-tection of apoptotic renal cells was carried out using TUNEL .The expression of Bcl-2 and Bax were observed by immunohis-tochemical method and activity of caspase-3 were measured with colorimetric chromatometry and .Results Compared with control group, the AI and expression of Bax decreased and Bcl-2 increased in treatment group at 8 h, 24 h, 48h and had statistical differences(P<0.01) .Caspase-3 activity decreased at 2 h, 8 h, 24 h, 48 h and had statistical differences(P<0.01).Conclusion The early application of z-VAD-fmk could effectively reduce renal cell apoptosis and relieve kidney damage.
Keywords：z-VAD-fmkcaspase-3Bcl-2BaxAIrats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 14-16 )