Interaction of lymphatic endothelial cell and cell line of nasopharyngeal carcinoma in vitro
ZHOU Xun-zhao
WU Yuan
ZHONG Chang-tao
YAN Kui
WEI Zheng-bo
XIE Ying
Abstract：Objective To investigate the interaction of highly metastatic cell line of nasopharyngeal carcinoma (5-8F) and lymphatic endothelial cell (LEC) in vitro.Methods The conditioned medium of 5-8F cell (5-8FCM) and LEC (LECCM) were prepared respectively .Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of 5-8FCM in different concentrations on the proliferation of LEC .Scratch wound healing assay was performed to observe the effect of LECCM in different concentrations on migration of 5-8F cell.The RPMI-1640 medium in different concentrations were acted as the control group in both experiments .Results The result of MTT assay showed that no statistical difference of cellular proliferation was found when comparing the various 5-8FCM concentration groups with their corresponding control groups af-ter cultured 24 h.After cultured for 48 h, the proliferation of LEC was facilitated in 5-8FCM group with 10%, 50%, 75%and 100%concentration levels (P<0.05).After 72 h, the proliferation of LEC was promoted in 5-8FCM group with con-centration levels of 75%and 100%( P<0.05) .The result of scratch wound healing suggested that the migratory distance of 5-8F treated with LECCM of each concentration was completely longer than the corresponding control group (P<0.05) after cultured for 6, 12 and 24 h.Conclusions The 5-8FCM could promote the proliferation of LEC and the LECCM could significantly increase the migration of 5-8F in vitro.The interaction between 5-8F and LEC could collectively promote the metastasis of nasopharyngeal carcinoma .
Keywords：nasopharyngeal carcinomalymphatic endothelial cellconditioned mediuminteraction
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )