Effect of BMP2 on mesenchymal stem cells differentiating into hepatocytes
WEN Biao
ZHOU Shu-xian
Abstract：Objective To observe the effect of BMP2 on bone marrow mesenchymal stem cells (BMSCs) differentia-ting into hepatocytes .Methods BMSCs in thighbone of rats were isolated and cultured using adherent culture method . The third generation of amplification of BMSCs in vitro was made into cell climbing piece , and directional inducting hepato-cyte.BMSCs was divided into Hepatocyte growth factor (HGF) group , HGF +BMP2 group, BMP2 group and blank con-trol group depending added inducing factor .Cells were collected after culturing about 10 days.Changes in cell morphology was observed .Using enzyme-linked immunosorbent assay to culture supernatant of liver cell-specific marker alpha-fetopro-tein (AFP), albumin (ALB), Cytokeratin immunohistochemistry assay cells -18 (CK-18).Results ALB, AFP and CK-18 in HGF group and HGF +BMP2 group can be detected , and were higher than those in HGF +BMP2 group ( all P<0.05).Conclusion BMP2 can not alonely induce BMSCs differentiating into hepatocytes ,enhance the effect of HGF-in-duced hepatocyte differenting into BMSCs .
Keywords：bone morphogenetic proteinsmyeloid progenitor cellgrowth substancehepatocytes
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 23-25 )