Expression of Peroxisome proliferator-activated receptor-γin hypoxia damage of renal tubular epithelial cells and its significance
JIANG Ling
LI Zheng-yi
ZHOU Zhi-qiang
CHEN Xiu-ping
LEI Feng-ying
QIN Yuan-han
Abstract：Objective To explore the expression and effects of Peroxisome proliferator-activated receptor-γ( PPARγ) in hypoxic damage of renal tubular epithelial cells (RTEC).Methods Rat's proximal tubular epithelial cell line (NRK-52E) was subcultured in vitro and randomly assigned into normal group, hypoxia model group, Rosiglitazone (RGZ) group and GW9662 group.RGZ (15μmol/L) and GW9662 (25μmol/L) were administered to RGZ group and GW9662 group respectively.The hy-poxia model group, RGZ group and GW9662 group were put into vacuum tank to establish the model of hypoxia/reperfusion-in-duced RTEC injury.The normal group did not make any processing.The expressions (mRNA and protein) of PPARγand trans-forming growth factor-β1(TGF-β1) in RTEC were detected by RT-PCR and Western blot 36 h after establishing models.Results Compared with normal group, the mRNA and protein expressions of PPARγin the hypoxia model group, RGZ group and GW9662 group were decreased;The expressions of PPARγin the RGZ group was elevated, and the indicator was lowered in the GW9662 group when compared with hypoxia model group (all P<0.05).Compared with normal group, the mRNA and protein expressions of TGF-β1 in the hypoxia model group, RGZ group and GW9662 group were increased;The expression of TGF-β1 in the RGZ group was decreased , the level of the indicator in the GW9662 group was increased when comparing with hypoxia model group (all P<0.05).Correlation analysis showed that the protein expression of PPARγwas negatively correlated with TGF-β1 in NRK-52E cell(P<0.05).Conclusion The lowered expression of PPARγmay be involved in hypoxia damage of RTEC;RGZ may alleviate the injury by elevating the expression of PPARγ.
Keywords：cell injurytubular epithelialperoxisome proliferator-activated receptor-γtransforming growth factor betarat
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )