The connection among the expression of p-tau protein and apoptosis related proteins Bcl-2 and Bax in the hippocampus of hypoxic-ischemic neonatal rats
WANG Jun
CHEN Jing
ZHANG Dong-xiu
WANG Bao-zhen
ZHU Deng-na
NIU Guo-hui
CHEGN Ping-ping
Abstract：Objective To observe the expression of phosphorylated tau ( p-tau) protein, and apoptosis related pro-teins Bcl-2 and Bax, and explore their correlations .Methods Seven-day-old newborn clean Sprague Dawley ( SD) rats, were randomly divided into normal control group (8 cases), sham operation (8 cases), and HIBD group (which was divid-ed into by death time points 3, 6, 12, 24, 48 h subsection 8 cases in each group).HIBD animal model was established by Rice method, and took the brain at different time points .In sham group, rats were just exposed the left carotid artery , and in the control group rats did not dispose .Rats were all decapitated brain immediately at the end of surgery .Hematoxylin-e-osin ( HE) staining was used to view morphological changes in the hippocampus , and immunohistochemical staining was used to test the expressions of p-tau, Bcl-2 and Bax protein.Results Brain tissues in the normal control group and sham operation group was normal .In HIBD group, the cell has not significant change 3 h after HIBD;Cell swelling and structur-al disorder 6 h after HIBD; Cell swelling significantly , nuclear condensation , fragmentation 24 h after HIBD;48 h after HIBD, apoptotic bodies occured , the number of nerve cells was dropped .In HIBD group, Bcl-2 and Bax positive cells in HIBD group at each time point were more than those in the normal control group and the sham group (all P<0.05);In the hippocampus of HIBD group , p-tau positive cells was positively correlated with Bcl-2 and Bax positive cells during 12 h af-ter HIBD(P<0.05).Conclusion The hippocampus neurolcytes actiwated apotosis mechanism afer HIBD early period . P-tau protein was involved in neuronal apoptosis after HIBD , and there is connection among p-tau, Bcl-2 and Bax protein.
Keywords：hypoxic-ischemiabrainp-tau proteinapoptosisBcl-2Baxrat
Publication Date：2014-12-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )