Expression and significant role of PHB1 and PHB2 in unilateral ureteral obstruction rats with renal tubulointerstital fibrosis
LEI Feng-yinga
CHEN Xiu-ping
ZHOU Zhi-qiang
HOU Tian-biao
HUANG Wei-fang
QIN Yuan-han
JIANG Ling
Abstract：Objective To investigate the expression and the role of PHB1 and PHB2 in unilateral ureteral obstruction (UUO) rats with renal tubulointerstital fibrosis (RIF).Methods Eighty Wistar rats (6 week-old) were randomly divided into model group (n=40) and sham operation group (SHO) (n=40).The left ureter of rats in model group were dissocia-ted to be obstructed,while were dissociated left ureter only in SHO.At the end of the 2th and 4th weekend, 20 rats in each group were sacrificed for histological and pathological examination of left kidney.RIF index was assessed according to patho-logical results.The mRNA expression of PHB1, PHB2 and TGF-β1 were detected by RT-PCR.Western blot was performed to detect the protein expression of PHB1, PHB2, TGF-β1, collagenⅣ(Col-Ⅳ), fibronectin (FN).Results Compared to SHO group, RIF index significantly increased in each time port in model group,and the longer the obstruction, the higher the RIF index all P<0.01;significantly lower expression of PHB1 and PHB2 mRNA and protein were observed in model group, the longer the obstruction, the lower expression lever all P<0.01;significantly increased the protein expression of TGF-β1 , Col-Ⅳand FN in model group (all P<0.01).PHB1 and PHB2 protein had a negative correlation with RIF, TGF-β1, Col-Ⅳ, FN protein (all P<0.05);PHB1 protein had a positive correlation with PHB2 (P<0.05).Conclusion Low expres-sion of PHB1 and PHB2 in UUO rats with RIF, which might be involved in the progression of RIF.
Keywords：renal interstitial fibrosisPHB1PHB2collagen-Ⅳfibronectinrat
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 37-39 )