Protective effect on pancreas isletβcells of NOD mouse derived from adenovirus vector-mediated murine IL-10 genes in early phase of type 1 diabetes mellitus

LI Cheng; LIN Xiao-jie; CHEN Yan-yan; ZHANG Li-juan; LI Tang

doi:10.3969/j.issn.1002-266X.2014.43.004

Abstract：Objective To obverse the protective effect on pancreas islet βcells of nonobese diabetic ( NOD) mouse in early phase of type 1 diabetes mellitus caused by adenovirus vector-mediated murine IL-10( Ad-mIL-10) genes.Methods 12 female NOD mice of 17-20 weeks old with diagnosis of type 1 diabetes mellitus less than 3 days were collected and random-ly divided into group A and B ( 6 mice in each group ) .Mice in group A were intraperitoneal injected with Ad-mIL-10 (0.1 mL) immediately in the early stage of diabetes and those in group B were treated with nothing as control .In addition, 6 NOD mice which in same age range and at inactive stage of disease were selected as group C , without any intervention .Data of weight, urine and blood sugar of mice at 4 time points which before the trial and 1, 2, 3 weeks after beginning were recor-ded for analysis .All mice were condemned to death in the third week , and whole blood was collected by method of picking eyeball, which then was used for measurement of IL-10, IFN-γ, IL-4 and C-peptide in blood serum.Several pathological sec-tions from pancreas which stained with HE and Immunohistochemistry assay were collected to analyze the infiltration degree of pancreas islet and expression of IL-10 genes in pancreas .Results Compared with group B , group A showed high IL-10 ex-pression in local pancreas and increased IL-10 figures in blood serum(P<0.01).In group A, the data of measured IFN-γand IFN-γ/IL-4 were lower than those of group B , whereas, IL-4 and IL-10 were detected in higher expression ( all P<0.01).C-peptide level in serum was more significant in group A (P<0.01).Conclusions IL-10 gene intervention has an immunomodulatory role on spontaneous disease of NOD mice .It can improve the function of islet beta cells to a certain ex-tent, but has no effect on insulitis alleviation and blood glucose control .