Correlation between microsatellite polymorphism in promoter of CDC37 and its expression in tissues of HBV-related HCC
CHEN Chao-wu
LIU Yu
XU Ying
YANG Gui-feng
WANG Zhan-hui
JIANG Rong-long
Abstract：Objective To explore the relationship between the microsatellite polymorphism in the promoter of CDC 37 and its expression in tissues of HBV-related HCC.Methods Genomic DNA was isolated from tissues of tumor and non-tumor of 22 patients with HBV-related HCC.Then the DNA was amplified using PCR to span the microsatellite of the hu-man CDC37 promoter.PCR products were cloned into PMD-18T vector.At least 3 clones of each specimen were se-quenced.The types and frequencies of(GT)n repeats were compared between:tumor and non-tumor of all patients;tumor and non-tumor of patients with CDC37 over-expression; tumor with and without CDC37 over-expression.Results Many types of microsatellite(GT)n1-GC-(GT)n2 were found in CDC37 gene promotor in tumor and non-tumor of the patients.The microsatellite was located at nt 1 368 to nt 1 415 of CDC37 promoter.The(GT)10-GC-(GT)9 was the most prevalent type. The distribution of the types of ( GT ) n repeats showed no difference between tumor and non-tumor of all patients ( P >0.05).No statistical difference was also found between tumor and non-tumor of patients with CDC37 over-expression(P>0.05).Significant difference was found between tumor with and without CDC 37 over-expression(P<0.05).The (GT)10-GC-( GT) 9 repeats showed a higher frequency in tumors with CDC 37 over-expression than in tumors without CDC 37 over-ex-pression(P<0.05), while the (GT)10-GC-(GT)12 repeats showed a higher prevalence in tumors without CDC 37 over-ex-pression comparing with tumors with CDC37 over-expression(P<0.05).Conclusions Microsatellite polymorphism is found in the promoter of CDC37 gene in tissues of tumor and non-tumor of patients with HBV-related HCC.The distribution of the types of (GT)n repeats shows statistical difference between tumor with and without CDC 37 over-expression.
Keywords：hepatitis B virushepatocellular carcinomaCDC37 geneCDC37 gene promotergene microsatellite
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )