Establishment of rat C6 glioma model engineered by green fluorescent protein
GAO Ge
NIU Chao-shi
ZHANG Jun
DONG Yong-fei
LI Dong-xue
FEI Xiao-rui
DING Wan-hai
Abstract：Objective To establish the rat C6 glioma model engineered by green fluorescent protein and explore its effect.Methods C6 cells were transfected with pEGFP-C1 plasmid expressing enhanced green fluorescent protein ( EG-FP) gene.Then the transfected cells were selected by G418 to obtain the stable C6 cell clones ( pEGFP-C1-C6) .pEGFP-C1-C6 cell suspension was injected into intracal of 16 SD rats to establish rat C6 glioma model with green fluorescent protein by stereotaxic apparatus.After inoculation, cell life cycle was observed and tumor growth status was dynamically documen-ted by magnetic resonance imaging.At 7, 14, 21 day after glioma model established, glioma tumor volumes were detected and measured by MRI.Brain specimens were observed by HE staining.Confocal laser scanning microscopy was used to ob-serve the infiltration and invasion of glioma cell.Results Rat C6 glioma model with green fluorescent protein was estab-lished successfully.Tumor cells invasion can be easily detected by green fluorescent protein imaging and MRI.Tumor vol-ume was (244.60 ±36.06)mm3.It was easier to observe the micro-metastasis and invasive.Conclusions Rat C6 glioma model could be successful established with EGFP-C1-C6 cells, which was more sensitive in showing invade, metastasis and single invasive tumor cell.
Keywords：green fluorescent proteingliomaanimal model
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 26-28 )