Protective efficacy of HO-1 plasmid loaded Lf-PEG-QMC brain targeting nanoparticles on hippocampal neurons in cerebral ischemic injury rats

ZHAO Zi-ming; QI Da-shi; SUN Ying; ZHANG Lei; LIU Yong-hai; LIU Hong-zhi

doi:10.3969/j.issn.1002-266X.2014.42.10

Abstract：Objective To investigate the protective efficacy of HO-1 plasmid loaded Lf-PEG-QMC nanoparticles on the hippocampal neurons of rats with cerebral ischemic injury.Methods A total of 24 adult SD rats were randomly divided into 4 groups: pseudo-operating group, ischemia-reperfusion injury group, ischemia-reperfusion injury +HO-1 plasmid group ( plasmid group ) , andischemia-reperfusion injury +HO-1 plasmid loaded Lf-PEG-QMC nanoparticles group ( NPs group) .Models of cerebral ischemia were established by the method of four vessels occlusion in ischemia-reperfusion injury group, plasmid group and NPs group.After the common carotid arteries were occluded for 15 minutes, reperfusion was con-ducted by vein in each group as 20 μg/kg HO-1 plasmids administered in plasmid group, HO-1 plasmid loaded Lf-PEG-QMC nanoparticles administered in NPs group.Rats in pseudo-operating group did not adopted the reperfusion.After 5 days of ischemia-reperfusion, rats brain in each group were extracted to make histologic sections.The survival statuses of neurons in hippocampal CA1 sector of each rats were detected by HE staining method and to calculate the survival neurons rate.Results The survival neurons rate of pseudo-operating group, ischemia-reperfusion injury group, plasmid group and NPs group was 100%, 11.0%, 16.6%, 57.6%, respectively.The survival neurons rate of ischemia-reperfusion injury group, plasmid group and NPs group was lower than that of pseudo-operating group(P<0.05).The survival neurons rate of NPs group was higher than that of ischemia-reperfusion injury group and plasmid group(P<0.05).There was no statis-tic difference between ischemia-reperfusion injury group and plasmid group.Conclusions Lf-PEG-QMC nanoparticles was effective in the transfer of HO-1 plasmid across BBB and it's stable express in brain, which indicates that Lf-PEG-QMC nanoparticles was helpful in the effect of HO-1 on neurons in cerebral ischemic injury.