Effect of protein kinase C inhibitors on the chemotherapeutic drug resistance of cervical carcinoma cell
WANG Jiao
YAN Hong-chao
Abstract：Objective To study the effect of protein kinase C inhibitors combined with paclitaxel on proliferation, apopto-sis, kinase C and P-glycoprotein (P-gp) expression of cervical cancer cell, in order to explore the effect of protein kinase C in-hibitor on the drug resistance of cervical cancer.Methods Cervical cancer Hela cells were cultured and divided into 4 groups, which were staurosporin group, paclitaxel group, combination group and control group.Staurosporin group were cultured with 0.6μmol/L staurosporin.Paclitaxel group were cultured with 0.1 μmol/L paclitaxel.Combination group were cultured with 0.6μmol/L staurosporin and 0.1μmol/L paclitaxel.Control group did not culture with staurosporin and paclitaxel.At 48 hours lat-er, the cell proliferation of each group was performed by MTT method;the cell apoptosis were detected by flow cytometry(FCM) method;the expression of protein kinase C and P-gp were detected by western-blot method.Results The proliferation of stau-rosporin group, paclitaxel group, combination group was 38.11%±0.70%, 49.96%±1.60%and 60.31%±1.80%, respec-tively.The proliferation of combination group was higher than that of staurosporin group and paclitaxel group(P<0.05).The proliferation of staurosporin group was lower than that of paclitaxel group(P<0.05).The cell apoptosis of control group, stauros-porin group, paclitaxel group and combination group was 7.13%±0.56%, 17.73%±0.59%, 36.51%±0.34%and 56.72%± 0.68%, respectively.The cell apoptosis of staurosporin group, paclitaxel group and combination group was higher than that of control group(P<0.05).The expression of protein kinase C and P-gp in staurosporin group and combination group were lower than those of control group(P<0.05).The expression of protein kinase C and P-gp in combination group were lower than those of staurosporin group and paclitaxel group(P<0.05).Conclusions The protein kinase C inhibits staurosporin can inhibit the proliferation of cervical cancer cells as well as induce its apoptosis, which means that staurosporin can improve the drug resist-ance of cervical cancer cells to paclitaxel.Staurosporin may invert the drug resistance in cervical cancer by reducing P-gp protein expression.
Keywords：cervical cancerprotein kinase C inhibitormulti-drug resistance gene 1paclitaxel
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 20-22 )