Culture and identification of adipose mesenchymal stem cells from diabetic patients in vitro
SHI Li
ZHANG De-ming
REN Si-po
HAN Guang-yu
TAN Kun
GENG Yue-chun
LIANG Jun
Abstract：Objective To establish the isolation and culture method of adipose mesenchymal stem cells derived from diabetic patients in vitro, so as to observe biological properties.Methods The ADSCs were isolated by digestion with col-lagenase from adipose tissue of diabetic patients, then cultured and purified in vitro.The cells were observed by inverted microscopeand.The P3, P6, P8 generation cells were collected and analyzed the cell growth curve.Then the cell surface markers and karyotype analysis of the P3 generation cells were detected.Their differentiation potential was proved by adipo-genic differentiation.Oil red staining positive proved they were fat cells.Results After isolation and culture, we obtained the ADSCs, which could passage stably and proliferate highly.The flow cytometry examination showed that the expression of CD34 and CD45 were negative, CD29 , CD90 , CD44 and CD105 were positive.Oil red staining was positive.Conclusion The ADSCs could be isolated from diabetic patients adipose tissue and be functionally induced into adipocytes in the pres-ence of appropriate conditioned media.
Keywords：diabetes mellitusadipose tissue-derived stromal cellsdifferentiation potential
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )