Anti-thrombosis effects of aspirin, clopidogrel and cilostazol

REN Geng-pu; LIU Yi-qing; ZHANG Bing-chang

doi:10.3969/j.issn.1002-266X.2014.42.06

Abstract：Objective To explore the anti-thrombosis effects of aspirin, clopidogrel, cilostazol.Methods A total of 70 SD rats were involved in this study and equally divided into 7 groups with 10 rats in each group.Rats in control group did not deal with any special processe.Rats in model group, aspirin group, clopidogrel group, cilostazol group and triad group were made coronary artery microembolization model, then rats in above groups were drenched 7 days with placebo, aspirin, clopidogrel, cilostazol and aspirin+clopidogrel+cilostazol, respectively.Abdominal aortic blood PT, APTT and TT were detected by automatic blood coagulation analyzer.Maximum agglutination of platelet was detected under the induce of ADP by the platelet aggregation rate tester.Blood cAMP, TXA2 and PDEⅢlevel were tested by the method of ELISA. After the blood test, all rats in each group were anaesthetized at dorsal decubitus to detect the formation of thrombus sup-pression rate by polyethylene tube method through left external jugular vein and right common carotid artery.Results Compared with model group, some observe indicator of 4 drug groups significant changed, including PT, APTT and TT pro-longed, maximum agglutination of platelet decreased, formation of thrombus suppression rate increased, blood cAMP level increased, TXA2 and PDEⅢdecreased ( all P<0.01) .Coagulation parameters of sham group, aspirin group, clopidogrel group, cilostazol group and triad group were no significance.Conclusions Aspirin, clopidogrel and cilostazol can also in-hibit platelet aggregation, reduce thrombosis, which may be related to the decrease of platelet aggregation, up-regulate the blood cAMP, down-regulate TXA2 and PDEⅢ.